Today, they are a very useful reproduction, reagent, and tool in various scientific disciplines, including mathematics and theoretical physics, biophysics, chemistry, colloid science, biochemistry, and biology. Sulfolobus and thermoplasma species have unique membranespanning tetraether lipids tel, which form stable liposomes. Liposomes were first reported by bangham and his coworkers in 1964 1. The incorporation of tetraether lipids into the liposomes does also not influence the high encapsulation efficiency. Archaeal lipids and their biotechnological applications. Twophoton fluorescence microscopy studies of bipolar. Oral delivery of vancomycin by tetraether lipid liposomes. Low permeability of liposomal membranes composed of. If you are viewing this page on a mobile device or would like to print this information for offline use, we have formatted the data into an easy to read pdf. The passive leakage of small molecules across membranes is a major limitation of liposomal drug formulations.
Since then, liposomes have made their way to the market. Abstract liposomes are lipid based vesicular systems that offer novel platform for versatile drug delivery to target cell. An extensive purification protocol to obtain a polar liposomalforming tetraether lipid fraction was reported by lo and chang 10. Stable archaeal tetraether lipid liposomes for photodynamic. Among the bipolar tetraether lipids, the polar lipid fraction e plfe is the main constituent lo and. For this purpose, the glycopeptide antibiotic vancomycin was used as model substance and liposomes were prepared by dac dual assymetric centrifugation. Conventional liposomes have a low level of stability in storage and are acidlabile. The lipids of archaea, based on glycerol isopranoid ethers, can be used taxonomically to distinguish between phenotypic subgroups of the domain to delineate them clearly from all other organisms. Cd and all other chemicals and solvents used were of analytical grade and were purchased from sigmaaldrich taufkirchen, germany. Biomimetic surface modification with bolaamphiphilic. Stability and protonpermeability of liposomes composed of. Applications of liposomes dr baumann cosmetics canada.
The modifications aim to enhance both the stability and the absorption of the liposomes. Tetraether lipid liposomes for the preparation of novel. This shortens the circulation times of the liposomes substantially. Us6316260b1 tetraether lipid derivatives and liposomes. Khan and parkson leegau chong department of biochemistry, temple university school of medicine, philadelphia, pennsylvania 19140 usa abstract membrane packing and dynamics of bipolar tetraether liposomes composed of the polar lipid fraction e plfe. The proper formulation for any particular application depends on many factors therefore no one formulation can be recommended. The leakage rate of trapped 5,6carboxyfluorescein 56cf and the proton permeability in plfe liposomes have been measured using fluorescence probe techniques in the. Us6316260b1 us09294,035 us29403599a us6316260b1 us 6316260 b1 us6316260 b1 us 6316260b1 us 29403599 a us29403599 a us 29403599a us 6316260 b1 us6316260 b1 us 6316260b1 authority us united states prior art keywords lipid tetraether derivative liposome group consisting prior art date 19970822 legal status the legal status is an assumption and is not a legal. Aug 30, 2011 oral peptide delivery by tetraether lipid liposomes. Liposomes can be stabilised with tetraether lipids, which are typical components of the membrane from different archae bacteria, the most. Here, we evaluate the leakage of 3 clinically used chemotherapeutic agents cytarabine, methotrexate and vincristine encapsulated in liposomes comprised of a synthetic, archaeainspired, membranespanning tetraether lipid. Liposomes and archaeosomes were prepared from the extracted lipid by thin film method. The liposome can be used as a vehicle for administration of nutrients and pharmaceutical drugs.
Compressibilities and volume fluctuations of archaeal. Archeobacteria membranes have diether andor tetraether linkages that are used for generation of lipid layers of archeosomes. Low permeability of liposomal membranes composed of bipolar. A liposome is a spherical vesicle having at least one lipid bilayer. They are rapidly cleared from the circulation by the macrophages which are located mainly in the liver, spleen, and bone marrow. Transfection studies with colloidal systems containing highly. Compressibilities and volume fluctuations of archaeal tetraether liposomes parkson leegau chong, michael sulc, and roland winter department of biochemistry, temple university school of medicine, philadelphia, pennsylvania. Purification and characterization of a liposomalforming. Photodynamic therapy hypericin tetraether liposome. Potential applications include novel lubricants, genedelivery systems, monolayer lipid matrices for sensor devices, and protein stabilization. Results given in table 2 demonstrated that lipid composition of the prepared liposomes and archaeosomes are very similar to the initial lipid compositions.
When preparing liposomes with mixed lipid composition, the lipids must first be dissolved and mixed in an organic solvent to assure a homogeneous mixture of lipids. There are many possible lipid formulations suitable for liposome preparations. A novel approach is presented here with the use of tetraether lipidcontaining liposomes. Since then, liposomes have undergone extensive research with the prime aim to optimize encapsulation, stability, circulation time and. Ple lipids were isolated as a single fraction from the crude lipid extract of s. These data suggest these liposomes retain the rigidity of the cytoplasmic membrane of s.
Studies of archaebacterial bipolar tetraether liposomes by. Liposomes can be prepared by disrupting biological membranes such as by sonication liposomes are most often composed of phospholipids, especially phosphatidylcholine, but may also include other lipids, such as egg. Accepted manuscript of archaeal lipids in oral delivery of therapeutic. Request pdf oral peptide delivery by tetraether lipid liposomes the aim of this study is to improve of oral peptide delivery by a novel type of liposomes containing tetraether lipids tels. Polar lipid e, ple, a native tetraether lipid mixture from sulfolobus acidocaldarius is shown to spontaneously form multilamellar liposomes in aqueous media. The data provide a deeper understanding of membrane packing and a molecular explanation for low solute permeation 15,16,19 and slow membrane dynamics 27,28 in plfe liposomes. A functional reconstitution of membrane proteins in monolayer liposomes from bipolar lipids of sulfolobus acidocazdarius received for publication, july 26, 1991. Wo1999010337a1 tetraether lipid derivatives and liposomes. Liposomal drugdelivery systems have been used for delivery of drugs to targeted tissues while reducing unwanted side effects. Tetraether lipid derivatives and liposomes and lipid agglomerates containing tetraether lipid derivatives, and use thereof.
Reconstitution of bacteriorhodopsin and atp synthase from micrococcus luteus into liposomes of the purified main tetraether lipid from thermoplasma acidophilum. Mechanisms by which nanoparticles alter the induction of immune responses. Preparation of liposomes and archaeosomes the lipid extraction of e. Request pdf on may 10, 2000, hansjoachim freisleben and others published tetraether lipid liposomes find, read and cite all the research you need on researchgate. A novel approach is presented here with the use of tetraether lipid containing liposomes. Such liposomes may offer new options for an oral delivery of peptides and protein drugs. With increasing temperature, the stability decreased and the proton permeability increased for all liposomes.
It also relates to the use of a tetraether lipid according to the invention and liposomes which contain 0. The journal of biological chemistry gi 1992 by the american society for biochemistry and molecular biology, inc. Characterization of drug encapsulation and retention in. Studies of archaebacterial bipolar tetraether liposomes by perylene fluorescence tapan k. Usually this process is carried out using chloroform or chloroform. A polar lipid fraction containing tetraether lipids caldarchaeol gdgt. A ulv liposome, b mlv liposome, c conventional pl liposome, d mixed vesicle and e tetraether lipid. Differential scanning calorimetry dsc and pressure perturbation calorimetry ppc were used to characterize thermal phase transitions, membrane packing, and volumetric properties in multilamellar vesicles mlvs composed of the polar lipid fraction e plfe isolated from the thermoacidophilic archaeon sulfolobus acidocaldarius grown at different temperatures. Transfection studies with colloidal systems containing. In the lipid mixing experiment, liposomes made of pa lipids labelled with a combination of 0.
The immunostimulatory activity of nanocarriers such as liposomes, archaeosomes and virosomes depends on diverse mechanisms. Nov 19, 2015 in the past decade, there is an emerging trend in the field of enhancing oral delivery of liposomes by modification of either the liposomal surfaces or the liposomal compositions. Tetraether lipid liposomes for the preparation of novel liposomal. Both compressibilities and volume fluctuations of plfe liposomes are much less temperaturesensitive than those in dppc liposomes. Longstanding discussions about pentacyclization of tetraether lipids. Membranelike lipid coatings open a wide field of various applications such as preparation of biosensor platforms, 1,2 1. Pdf temperature and phdependent molecular dynamics of. Thioltriggered release of intraliposomal content from. The absence of a lipid phase transition or aggregationfusion of liposomes near room temperature suggests that the gmgtpc lipids are suitable for evaluating the effects of small rings incorporated into tetraether lipids on membrane leakage. Oral delivery of vancomycin by tetraether lipid liposomes request.
Applications of liposomes 499 although they are composed from natural substances liposomes are no exception. Jan 28, 2019 oral delivery of vancomycin by tetraether lipid liposomes. Tetraether lipid derivatives and liposomes and lipid agglomerates containing tetraetherlipid derivatives, and use thereof. However, while this liposomal formulation allows for a morestable circulation of. Lipid vectors are commonly used to facilitate the transfer of nucleic acids into mammalian cells. Artificial lipid membrane systems are used to mimic natural membrane properties in terms of stability and homogeneity. Liposomes composed from tetraether lipids, however, remain the most stable. The inventive tetraetherlipid derivatives are very stable and are well suited to lipofection. Improved oral bioavailability of human growth hormone by a. Us6316260b1 tetraether lipid derivatives and liposomes and. Contents of these liposomes are most often destined for lysosomes new, 1990. In this study, two fractions of tetraether lipids from the archaea sulfolobus acidocaldarius were extracted and purified using different methods. Biomimetic surface modification with bolaamphiphilic archaeal.
These liposomes are often used for targeting of the reticuloendothelial system res. The stability assay performed in simulated gastric showed a high stability for the liposomal formulation containing tetraether lipids indicated by the liposomal size and pdi, which remained nearly constant over 5 h. Pdf reconstitution of bacteriorhodopsin and atp synthase. Fusion of bipolar tetraether lipid membranes without enhanced. Application of various types of liposomes in drug delivery. Doxil, for instance, is a liposomal formulation of the anticancer agent doxorubicin dox that has been used to address problems associated with nonspecific toxicity of free dox. In this work, we present a serumstable, cholesterolintegrated tetraether lipid comprising of a cleavable disulfide bond, gcgtsspcch, that is designed to respond to the reducing environment of the cell to trigger the release intraliposomal content upon cellular uptake by cancer cells. Conventional liposomes which are used for transporting pharmaceutical active agents in eukaryotic cells or for lipofection can only be preserved for a limited period, are not acidstable and require a number of set parameters in order to achieve satisfactory results. Fusion of bipolar tetraether lipid membranes without. Crude lipids were purified by subsequent reversedphase chromatography. Temperature and phdependent molecular dynamics of thermoplasma acidophilum tetraether lipid membrane in a computersimulated model. When this system is used for drug delivery, both hydrophilic and lipophilic drugs can be transported therein torchilin, 2007. Preparation and characterization of stealth archaeosomes.
The physical origin of the extremely high thermal stability of tetraether liposomes composed of the polar lipid fraction e plfe from the thermoacidophilic archaebacterium sulfolobus acidocaldarius has been investigated. Cyclohexane rings reduce membrane permeability to small. The methanolprecipitated mixture was able to form liposomes, whereas. The purified lipid fractions polar lipid fraction e plfe and hydrolysed glyceroldialkylnonitol tetraether hgdnt differ in their structures. Recently, we cultured thermoplasma species from the indonesian volcano. Less sensitive liposomes are therefore highly desirable. Tetraether lipids were isolated from archea species, which have extreme stable cell membranes stabile in low ph and high temperature mediums. The aim of this study is to improve of oral peptide delivery by a novel type of liposomes containing tetraether lipids tels derived from archaea bacteria. The inventive tetraetherlipid derivatives are very stable and are well. Liposomes, sphereshaped vesicles consisting of one or more phospholipid bilayers, were first described in the mid60s. Evaluation of tetraether lipidbased liposomal carriers for encapsulation and retention of nucleosidebased drugs. These liposomes are composed of natural phospholipids which may be neutral or negatively charged and cholesterol. We previously showed that liposomes made from pure archaeainspired bipolar tetraether lipids exhibit exceptionally low permeability of. As nouns the difference between lipid and liposome is that lipid is organic compound any of a group of organic compounds including the fats, oils, waxes, sterols, and triglycerides lipids are characterized by being insoluble in water, and account for most of the fat present in the human body they are, however, soluble in nonpolar organic solvents while liposome is.
In order to evaluate the relative permeability of membranes. Request pdf oral delivery of vancomycin by tetraether lipid liposomes despite the outstanding progress in modern medicine, the oral delivery of peptide drugs is limited until today due to. Oral peptide delivery by tetraether lipid liposomes. Liposomes comprised of the pure tetraether lipid exhibited superior retention of both a neutrally and positively charged drug up to an. The purified lipid fractions polar lipid fraction e plfe and hydrolysed glyceroldialkylnonitol tetraether hgdnt differ in their structures, charge. Dec 18, 2019 in the lipid mixing experiment, liposomes made of pa lipids labelled with a combination of 0. The intent is to obtain a clear lipid solution for complete mixing of lipids. To overcome these hurdles, liposomes containing the specific tetraether lipid gcte glycerylcaldityltetraether lipid were examined. These results were in good accordance with data generated by lipid experiments at the airwater interface applying a langmuirblodgett film balance so that the selfassembly potential of two different tetraether lipids was found to be sufficient to form stable liposomes in aqueous media. The effects of temperature and ph on laurdan 6lauroyl2dimethylaminonaphthalene fluorescence intensity images of giant unilamellar vesicles guvs. Request pdf oral delivery of vancomycin by tetraether lipid liposomes despite the outstanding progress in modern medicine, the oral delivery of peptide. Modes of liposome action liposomes as drug delivery systems can offer several advantages over conventional. The isolation and characterization of archaeal tetraether lipids has led to some interesting applications improving on ester lipid technologies. We have compiled a list of lipids commonly used in liposome research which provides a starting point.
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